Protein concentration of unprocessed VDTP preparation has been determined by Bradford assay (IB-16.05.00). After processing, VDTP final solutions were prepared in concentrations: 167 ng/ml; 1,000 ng/mI and 3,334 ng/mI (±10%) in relation to initial concentration.
In order to test whether components of Omnia inhibit cancer cells proliferation, we treated MCF-7 cells (cell line derived from human breast cancer) with albumin conjugated Omnia. Albumin is necessary as a transporter of oleic acid (under in vitro conditions). The assay used in the experiment is colorimetric cell proliferation assay, where absorbance at a specific wavelength is proportional to cells viability.
Anticancer efficacy of Vitamin D Transport Protein (VDTP) can be ascribed at least to three biological properties of the molecule: 1) activation of tumoricidal macrophages; 2) inhibition of tumor-induced angiogenesis and 3) direct inhibition of cancer cell proliferation, migration and metastatic potential.