Omnia & MCF-7 cells assay

Omnia & MCF-7 cells assay

Wednesday, Jul 12, 2017 0 comment(s)

In order to test whether components of Omnia inhibit cancer cells proliferation, we treated MCF-7 cells (cell line derived from human breast cancer) with albumin conjugated Omnia. Albumin is necessary as a transporter of oleic acid (under in vitro conditions). The assay used in the experiment is colorimetric cell proliferation assay, where absorbance at a specific wavelength is proportional to cells viability.

Oleic acid, one of Omnia Drops active components, exhibits ability to inhibit growth of various cancer cell lines.

Reference:

Li S, Zhou T, Li C, Dai Z, Che D, et al. (2014) High Metastaticgastric and Breast Cancer Cells Consume Oleic Acid in an AMPK Dependent Manner. PLoSONE 9(5): e97330. doi:10.1371/journal.pone.0097330

Results:

Fig. 1: Breast cancer cells viability after treatment with albumin conjugated oleic acid. After 72h incubation with 2mM albumin-conjugated Omnia cell viability was reduced to ≈ 54.5 % with respect to control.

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